大鼠核因子κB受体激活因子(RANk)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat RANk(Receptor Activator Of Nuclear Factor Kappa B) Microsample ELISA Kit","alternative_names":"CD265; TNFRSF11A; TNFRSF11-A; ODFR; OFE; TRANCER; Osteoclast differentiation factor receptor; Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB Activator","species":"Rat","assay_type":"Sandwich","sensitivity":"0.06 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Metabolic pathway;Bone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RANk. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RANk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RANk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RANk in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9ESE2","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5786MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5786MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5786MS",}