大鼠成肌分化蛋白(MyoD)微量上样酶联免疫吸附检测试剂盒

英文名称	Rat MyoD(Myogenic Differentiation) Microsample ELISA Kit
别名	MYOD1; PUM; MYF3; bHLHc1; Myoblast Determination Protein 1; Myogenic Factor 3; Class C basic helix-loop-helix protein 1

种属	Rat
检测类型	Sandwich
灵敏度	27 pg/mL
检测范围	78.13-5000 pg/mL
样本类型	Tissue homogenates, cell lysates and other biological fluids.
实验时长	3.5h

研究领域	Metabolic pathway;Cardiovascular biology;Developmental science;Bone metabolism;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MyoD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MyoD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MyoD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MyoD in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q02346

标准曲线图

【仅供科学研究使用】

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