小鼠泛醌细胞色素C还原酶核心蛋白Ⅱ(UQCRC2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse UQCRC2(Ubiquinol Cytochrome C Reductase Core Protein II) ELISA Kit","alternative_names":"QCR2; UQCR2; Cytochrome b-c1 complex subunit 2, mitochondrial; Complex III subunit 2; Core protein II","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.059 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse UQCRC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse UQCRC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse UQCRC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse UQCRC2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9DB77","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6054-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6054-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6054",}