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小鼠赖氨酸tRNA合成酶(KARS)酶联免疫吸附检测试剂盒

  • 货号:ELK6083
  • 价格:¥2,800.00 元
  • 点击:2362
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产品介绍

产品信息

英文名称Mouse KARS(Lysyl tRNA Synthetase) ELISA Kit
别名KARS2;  LysRS; Lysine tRNA Ligase

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Mouse
检测类型Sandwich
灵敏度13.1 pg/mL
检测范围31.25-2000 pg/mL
样本类型serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KARS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q99MN1

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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