小鼠肌苷三磷酸酶(ITPA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse ITPA(Inosine Triphosphatase) ELISA Kit","alternative_names":"ITPase; Nucleoside Triphosphate Pyrophosphatase; Inosine Triphosphate Pyrophosphatase; Non-canonical purine NTP pyrophosphatase; Nucleoside-triphosphate diphosphatase","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.047 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell Lysates and other biological fluids.","assay_length":"3.5h","research_area":"Metabolic pathway;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITPA in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9D892","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6231-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6231-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6231",}