大鼠神经生长因子诱导蛋白B(NGFIB)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat NGFIB(Nerve Growth Factor IB) Microsample ELISA Kit","alternative_names":"GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3; DHR38; TIS1; Orphan nuclear receptor HMR; Testicular receptor 3; Nuclear receptor subfamily 4 group A member 1","species":"Rat","assay_type":"Sandwich","sensitivity":"0.054 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Neuro science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NGFIB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NGFIB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NGFIB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NGFIB in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P22829","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6295MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6295MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6295MS",}