人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human IGF2BP3(Insulin Like Growth Factor 2 mRNA Binding Protein 3) Microsample ELISA Kit","alternative_names":"MP-3; IMP3; KOC1; VICKZ3; CT98; IGF II mRNA Binding Protein 3; Cancer\/Testis Antigen 98; KH domain-containing protein overexpressed in cancer","species":"Human","assay_type":"Sandwich","sensitivity":"0.068 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Cytokine;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGF2BP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGF2BP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGF2BP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGF2BP3 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O00425","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6376MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6376MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6376MS",}