人Nei内切核酸酶Ⅷ样蛋白1(NEIL1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human NEIL1(Nei Endonuclease VIII Like Protein 1) ELISA Kit","alternative_names":"NEI1; hFPG1; FPG1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Nei-like protein 1; DNA glycosylase\/AP lyase Neil1","species":"Human","assay_type":"Sandwich","sensitivity":"0.065 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NEIL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEIL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NEIL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEIL1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q96FI4","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6435-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6435-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6435",}