小鼠细胞色素P450家族成员3A4(CYP3A4)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse CYP3A4(Cytochrome P450 3A4) ELISA Kit","alternative_names":"CYP3A3; CYPIIIA4; 1,8-cineole 2-exo-monooxygenase; Albendazole sulfoxidase; Nifedipine oxidase; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.64 ng\/mL","standard":"100 ng\/mL","detection_range":"1.57-100 ng\/mL","sample_type":"Tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CYP3A4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CYP3A4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CYP3A4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CYP3A4 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6498-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6498-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6498",}