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人防御素β112(DEFb112)酶联免疫吸附检测试剂盒

  • 货号:ELK6531
  • 价格:¥2,800.00 元
  • 点击:1542

产品介绍

产品信息

英文名称Human DEFb112(Defensin Beta 112) ELISA Kit
别名DEFB-12

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Human
检测类型Sandwich
灵敏度29.5 pg/mL
检测范围78.13-5000 pg/mL
样本类型Serum, plasma and other biological fluids.
实验时长3.5h

研究信息

研究领域Immune molecule;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEFb112. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFb112. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DEFb112, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DEFb112 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q30KQ8

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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