小鼠DNA修复蛋白RAD50(RAD50)酶联免疫吸附检测试剂盒

英文名称	Mouse RAD50(DNA Repair Protein RAD50) ELISA Kit
别名	DNA repair protein RAD50; hRAD50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog; RAD50 PEN; Truncated RAD50 protein

种属	Mouse
检测类型	Sandwich
灵敏度	0.058 ng/mL
检测范围	0.16-10 ng/mL
样本类型	tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Signal transduction;Tumor immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RAD50. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RAD50. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RAD50, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RAD50 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P70388

标准曲线图

【仅供科学研究使用】

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