大鼠封闭蛋白5(CLDN5)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat CLDN5(Claudin 5) ELISA Kit","alternative_names":"AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS","species":"Rat","assay_type":"Sandwich","sensitivity":"0.117 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CLDN5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CLDN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CLDN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CLDN5 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9JKD6","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6593-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6593-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6593",}