人细胞程序性死亡蛋白5(PDCD5)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human PDCD5(Programmed Cell Death Protein 5) ELISA Kit","alternative_names":"TFAR19; TFAR19 Novel Apoptosis-Related; TF1 Cell Apoptosis-Related Gene 19","species":"Human","assay_type":"Sandwich","sensitivity":"0.057 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDCD5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDCD5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDCD5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDCD5 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O14737","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6633-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6633-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6633",}