大鼠甲状腺素受体α(THRa)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat THRa(Thyroid Hormone Receptor Alpha) Microsample ELISA Kit","alternative_names":"THR-A; NR1A1; AR7; EAR7; ERB-T1; ERBA1; NR1-A1; THRA1; THRA2; c-ERBA1; Erythroblastic Leukemia Viral Oncogene Homolog; Nuclear Receptor Subfamily 1,Group A,Member 1","species":"Rat","assay_type":"Sandwich","sensitivity":"0.269 ng\/mL","standard":"40 ng\/mL","detection_range":"0.63-40 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat THRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat THRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat THRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat THRa in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P63059","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6646MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6646MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6646MS",}