大鼠淋巴毒素β(LTb)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat LTb(Lymphotoxin Beta) ELISA Kit","alternative_names":"LT-B; p33; TNFC; TNF-C; TNFSF3; Tumor necrosis factor ligand superfamily member 3; Tumor Necrosis Factor C","species":"Rat","assay_type":"Sandwich","sensitivity":"6.3 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"Serum, plasma and other biological fluids.","assay_length":"3.5h","research_area":"Cytokine;Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LTb in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6660-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6660-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6660",}