小鼠Cerberus蛋白(CER)微量上样酶联免疫吸附检测试剂盒

英文名称	Mouse CER(Cerberus) Microsample ELISA Kit
别名	CER1; DAND4; Cerberus 1; DAN domain family member 4; Cysteine Knot Superfamily,Homolog(Xenopus Laevis); Cerberus-related protein

种属	Mouse
检测类型	Sandwich
灵敏度	11.5 pg/mL
检测范围	31.25-2000 pg/mL
样本类型	Serum, plasma and other biological fluids.
实验时长	3.5h

研究领域	Cytokine;Signal transduction;Developmental science;Bone metabolism;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CER in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O55233

标准曲线图

【仅供科学研究使用】

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