人蛋白L-异天冬氨酸-O-甲基转移酶(PCMT1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human PCMT1(Protein L-Isoaspartate-O-Methyltransferase) ELISA Kit","alternative_names":"PIMT; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl\/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase","species":"Human","assay_type":"Sandwich","sensitivity":"13.5 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCMT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PCMT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PCMT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCMT1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P22061","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6763-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6763-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6763",}