大鼠羟烷基辅酶A脱氢酶β(HADHb)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat HADHb(Hydroxyacyl Coenzyme A Dehydrogenase Beta) ELISA Kit","alternative_names":"MTPB; Mitochondrial Trifunctional Protein,Beta Subunit; 3-Ketoacyl-Coenzyme A Thiolase\/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit; Beta-ketothiolase","species":"Rat","assay_type":"Sandwich","sensitivity":"0.13 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HADHb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HADHb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HADHb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HADHb in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6779-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6779-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6779",}