大鼠细胞外基质磷酸糖蛋白(MEPE)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat MEPE(Matrix Extracellular Phosphoglycoprotein) Microsample ELISA Kit","alternative_names":"OF45; Asarm Motif-Containing; Osteoblast\/Osteocyte Factor 45","species":"Rat","assay_type":"Sandwich","sensitivity":"0.131 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;Bone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MEPE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MEPE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MEPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MEPE in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9ES02","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6848MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6848MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6848MS",}