小鼠L-型电压依赖性钙离子通道α1D亚基(CACNa1D)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse CACNa1D(Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit","alternative_names":"CACH3; CACN4; CACNL1A2; CCHL1A2; Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3; Calcium channel, L type, alpha-1 polypeptide, isoform 2","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.117 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CACNa1D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CACNa1D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CACNa1D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CACNa1D in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q99246","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6872-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6872-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6872",}