大鼠肝细胞核因子1β(HNF1b)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat HNF1b(Hepatocyte Nuclear Factor 1 Beta) Microsample ELISA Kit","alternative_names":"FJHN; TCF2; HNF1βeta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2,Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3","species":"Rat","assay_type":"Sandwich","sensitivity":"0.056 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Cytokine;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNF1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNF1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNF1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNF1b in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P23899","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK6882MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK6882MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK6882MS",}