大鼠肉毒碱棕榈酰基转移酶1A(CPT1A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat CPT1A(Carnitine Palmitoyltransferase 1A, Liver) ELISA Kit","alternative_names":"CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform","species":"Rat","assay_type":"Sandwich","sensitivity":"1.23 ng\/mL","standard":"200 ng\/mL","detection_range":"3.13-200 ng\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CPT1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CPT1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CPT1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CPT1A in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P32198","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7024-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7024-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7024",}