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牛70kDa热休克蛋白1B(HSPA1B)酶联免疫吸附检测试剂盒

  • 货号:ELK7059
  • 价格:¥2,800.00 元
  • 点击:2122

产品介绍

产品信息

英文名称Cattle HSPA1B(Heat Shock 70kDa Protein 1B) ELISA Kit
别名HSP70-2; HSP-A1B; Heat Shock Protein 72

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Cattle
检测类型Sandwich
灵敏度0.54 ng/mL
检测范围1.57-100 ng/mL
样本类型Tissue homogenates and other biological fluids.
实验时长3.5h

研究信息

研究领域Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HSPA1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HSPA1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HSPA1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HSPA1B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q27965

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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