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大鼠70kDa热休克蛋白1A(HSPA1A)酶联免疫吸附检测试剂盒

  • 货号:ELK7102
  • 价格:¥2,800.00 元
  • 点击:2155
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产品介绍

产品信息

英文名称Rat HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit
别名HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.57 ng/mL
检测范围1.57-100 ng/mL
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长3.5h

研究信息

研究领域Signal transduction;Tumor immunity;Infection immunity;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPA1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPA1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPA1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPA1A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P0DMW0

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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