大鼠脊椎蛋白2(SPON2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat SPON2(Spondin 2) ELISA Kit","alternative_names":"DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1","species":"Rat","assay_type":"Sandwich","sensitivity":"0.068 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Serum, plasma and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPON2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPON2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPON2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPON2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9WV75","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7130-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7130-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7130",}