大鼠乳脂球表皮生长因子8(MFGE8)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit","alternative_names":"HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8","species":"Rat","assay_type":"Sandwich","sensitivity":"5.8 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P70490","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7230-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7230-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7230",}