大鼠转录激活因子4(ATF4)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat ATF4(Activating Transcription Factor 4) ELISA Kit","alternative_names":"TAXREB67; TXREB; CREB-2; DNA-binding protein TAXREB67; Cyclic AMP-responsive element-binding protein 2; Tax-responsive enhancer element-binding protein 67","species":"Rat","assay_type":"Sandwich","sensitivity":"28 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Developmental science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATF4 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9ES19","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7264-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7264-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7264",}