犬单核细胞趋化蛋白2(MCP2)酶联免疫吸附检测试剂盒

英文名称	Dog MCP2(Monocyte Chemotactic Protein 2) ELISA Kit
别名	CCL8; CCL-8; SCYA8; MCP-2; HC14; Chemokine C-C-Motif Ligand 8; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 8; Monocyte Chemoattractant Protein 2

种属	Dog
检测类型	Sandwich
灵敏度	12.1 pg/mL
检测范围	31.25-2000 pg/mL
样本类型	Serum, plasma, tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Cytokine;Infection immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog MCP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog MCP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog MCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog MCP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q68AY9

标准曲线图

【仅供科学研究使用】

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