人DOT1样组蛋白H3甲基转移酶(DOT1L)酶联免疫吸附检测试剂盒

英文名称	Human DOT1L(DOT1 Like, Histone H3 Methyltransferase) ELISA Kit
别名	DOT1; KMT4; Histone-lysine N-methyltransferase, H3 lysine-79 specific; Histone H3-K79 methyltransferase; Lysine N-methyltransferase 4

种属	Human
检测类型	Sandwich
灵敏度	0.058 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates, cell lysates and other biological fluids.
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DOT1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DOT1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DOT1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DOT1L in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q8TEK3

标准曲线图

【仅供科学研究使用】

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