人丝织蛋白样蛋白(DBNL)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human DBNL(Drebrin Like Protein) Microsample ELISA Kit","alternative_names":"ABP1; HIP-55; SH3P7; CMAP; Cervical mucin-associated protein; Drebrin-F; HPK1-interacting protein of 55 kDa; SH3 domain-containing protein 7","species":"Human","assay_type":"Sandwich","sensitivity":"0.64 ng\/mL","standard":"100 ng\/mL","detection_range":"1.57-100 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DBNL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DBNL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DBNL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DBNL in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9UJU6","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7533MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7533MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7533MS",}