小鼠蛋白磷酸酶1调节因子亚基15A(PPP1R15A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse PPP1R15A(Protein Phosphatase 1, Regulatory Subunit 15A) ELISA Kit","alternative_names":"PPP1-R15A; PPP1R15-A; PPP1-R15-A; GADD34; Growth Arrest And DNA-Damage-Inducible 34; Myeloid differentiation primary response protein MyD116 homolog","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.057 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Developmental science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPP1R15A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPP1R15A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPP1R15A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPP1R15A in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7615-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7615-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7615",}