小鼠无翅型MMTV整合位点家族成员3A(WNT3A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse WNT3A(Wingless Type MMTV Integration Site Family, Member 3A) ELISA Kit","alternative_names":"Protein Wnt 3a Precursor; Protein Wnt-3a; Wnt 3a; wnt3a; WNT3A protein;","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.054 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse WNT3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse WNT3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse WNT3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse WNT3A in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P27467","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7664-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7664-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7664",}