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大鼠乳酸脱氢酶B(LDHB)酶联免疫吸附检测试剂盒

  • 货号:ELK7736
  • 价格:¥2,800.00 元
  • 点击:2481
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产品介绍

产品信息

英文名称Rat LDHB(Lactate Dehydrogenase B) ELISA Kit
别名LDH-B; LDH-H; Renal carcinoma antigen NY-REN-46

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.123 ng/mL
检测范围0.32-20 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Cardiovascular biology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LDHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LDHB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P42123

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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