大鼠饥饿素(GHRL)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat GHRL(Ghrelin) Microsample ELISA Kit","alternative_names":"MTLRP; Growth Hormone-Releasing Peptide","species":"Rat","assay_type":"Sandwich","sensitivity":"30 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GHRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9QYH7","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7752MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7752MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7752MS",}