小鼠巨噬细胞炎性蛋白1γ(MIP1g)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse MIP1g(Macrophage Inflammatory Protein 1 Gamma) ELISA Kit","alternative_names":"CCL9; CCL10; SCYB9; CCF18; MRP2; C-C motif chemokine 9; Macrophage inflammatory protein-related protein 2; Small-inducible cytokine A9","species":"Mouse","assay_type":"Sandwich","sensitivity":"3.2 pg\/mL","standard":"500 pg\/mL","detection_range":"7.82-500 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Cytokine;Infection immunity;Human gene deletion;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7798-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7798-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7798",}