5-羟色胺(5-HT)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"5-HT(5-Hydroxytryptamine) ELISA Kit","alternative_names":"5HT; Serotonin; N-Methyl-Gamma","species":"General","assay_type":"Competitive Inhibition","sensitivity":"3.94 ng\/mL","standard":"900 ng\/mL","detection_range":"14.07-900 ng\/mL","sample_type":"serum, plasma","assay_length":"2h","research_area":"Signal transduction;Infection immunity;Neuro science;Gastroenterology;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7830-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7830-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7830",}