1,5-脱水葡萄糖醇(1,5-AG)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"1,5-AG(1,5-Anhydroglucitol) ELISA Kit","alternative_names":"LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol","species":"General","assay_type":"Competitive Inhibition","sensitivity":"0.6 µg\/mL","standard":"150 µg\/mL","detection_range":"2.35-150 µg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"2h","research_area":"Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7840-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7840-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7840",}