氰钴胺素(CNCbl)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"CNCbl(Cyanocobalamin) ELISA Kit","alternative_names":"VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide","species":"General","assay_type":"Competitive Inhibition","sensitivity":"49.1 pg\/mL","standard":"10000 pg\/mL","detection_range":"156.25-10000 pg\/mL","sample_type":"serum, plasma, other biological fluids","assay_length":"2h","research_area":"Metabolic pathway;Nutrition metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CNCbl. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7871-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7871-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7871",}