反三碘甲状腺原氨酸(rT3)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"rT3(Reverse Triiodothyronine) ELISA Kit","alternative_names":"RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine","species":"General","assay_type":"Competitive Inhibition","sensitivity":"27.68 pg\/mL","standard":"6000 pg\/mL","detection_range":"93.75-6000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"2h","research_area":"Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7898-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7898-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7898",}