小鼠氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse AIMP1(Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1) ELISA Kit","alternative_names":"SCYE1; EMAP II; EMAP2; p43; Small Inducible Cytokine Subfamily E,Member 1; Endothelial monocyte-activating polypeptide II; Multisynthase complex auxiliary component p43","species":"Mouse","assay_type":"Sandwich","sensitivity":"11.7 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AIMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AIMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P31230","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7946-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7946-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7946",}