大鼠棘皮动物微管关联蛋白样蛋白2(EML2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat EML2(Echinoderm Microtubule Associated Protein Like Protein 2) ELISA Kit","alternative_names":"EMAP2; ELP70; Echinoderm MT-Associated Protein (EMAP)-Like Protein 70","species":"Rat","assay_type":"Sandwich","sensitivity":"0.13 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Metabolic pathway;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EML2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EML2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EML2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EML2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q6P6T4","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8021-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8021-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8021",}