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1,25-二羟基维生素D3(DHVD3)酶联免疫吸附检测试剂盒

  • 货号:ELK8091
  • 价格:¥2,400.00 元
  • 点击:1765

产品介绍

产品信息

英文名称DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit
别名DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属General
检测类型Competitive Inhibition
灵敏度48.8 pg/mL
检测范围156.25-10000 pg/mL
样本类型serum, plasma and other biological fluids
实验时长2h

研究信息

研究领域Metabolic pathway;Bone metabolism;
检测原理This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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