环磷酸腺苷(cAMP)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"cAMP(Cyclic Adenosine Monophosphate) ELISA Kit","alternative_names":"c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate","species":"General","assay_type":"Competitive Inhibition","sensitivity":"92.2 pg\/mL","standard":"20000 pg\/mL","detection_range":"312.5-20000 pg\/mL","sample_type":"serum, plasma, cell lysates, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Signal transduction;Metabolic pathway;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8116-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8116-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8116",}