磷脂酰肌醇三磷酸(PIP3)酶联免疫吸附检测试剂盒
- 货号：ELK8250
- 价格：￥2,800.00 元
- 点击：1992
产品介绍
产品信息
| 英文名称 | PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit |
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| 别名 | PtdIns(3,4,5)P3 |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | General |
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| 检测类型 | Competitive Inhibition |
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| 灵敏度 | 2.84 ng/mL |
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| 检测范围 | 7.82-500 ng/mL |
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| 样本类型 | serum, plasma and other biological fluids |
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| 实验时长 | 2h |
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研究信息
| 研究领域 | Signal transduction; |
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| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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