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猪B细胞活化因子(BAFF/CD257)酶联免疫吸附检测试剂盒

  • 货号:ELK8277
  • 价格:¥2,800.00 元
  • 点击:1079

产品介绍

产品信息

英文名称Pig BAFF/CD257(B-Cell Activating Factor) ELISA Kit
别名CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Pig
检测类型Sandwich
灵敏度0.057 ng/mL
检测范围0.16-10 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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