大鼠前心钠肽(Pro-ANP)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat Pro-ANP(Pro Atrial Natriuretic Peptide) Microsample ELISA Kit","alternative_names":"Pro-ANP","species":"Rat","assay_type":"Sandwich","sensitivity":"15.2 pg\/mL","standard":"4000 pg\/mL","detection_range":"62.5-4000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Endocrinology;Cardiovascular biology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P01161","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8360MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8360MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8360MS",}