小鼠4-羟基壬烯酸(4-HNE)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse 4-HNE(4-Hydroxynonenal) Microsample ELISA Kit","alternative_names":"4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal","species":"Mouse","assay_type":"Sandwich","sensitivity":"1.1 ng\/mL","standard":"150 ng\/mL","detection_range":"2.35-150 ng\/mL","sample_type":"Serum, plasma and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8374MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8374MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8374MS",}