小鼠肿瘤坏死因子受体超家族成员9(TNFRSF9)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Mouse TNFRSF9(Tumor Necrosis Factor Receptor Superfamily, Member 9) ELISA Kit","alternative_names":"CD137; CDw137; 4-1BB; ILA; Induced By Lymphocyte Activation; T-cell antigen 4-1BB homolog; CD137L","species":"Mouse","assay_type":"Sandwich","sensitivity":"6 pg\/mL","standard":"1500 pg\/mL","detection_range":"23.5-1500 pg\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"CD & Adhesion molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TNFRSF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TNFRSF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TNFRSF9 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P20334","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8418-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8418-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8418",}