{"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human SA(Sialic Acid) ELISA Kit","alternative_names":"SA","species":"Human","assay_type":"Competitive Inhibition","sensitivity":"3.83 µg\/mL","standard":"500 µg\/mL","detection_range":"7.82-500 µg\/mL","sample_type":"Serum, plasma, other biological fluids","assay_length":"2.5h","research_area":"Signal transduction;Tumor immunity;Hepatology;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SA in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8419-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8419-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8419",}