大鼠蛋白激酶Bα(PKBa)微量上样酶联免疫吸附检测试剂盒
- 货号：ELK8430MS
- 价格：￥2,600.00 元
- 点击：727
产品介绍
产品信息
| 英文名称 | Rat PKBa(Protein Kinase B Alpha) Microsample ELISA Kit |
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| 别名 | AKT1; AKT; PKB-A; Proto-oncogene c-Akt; RAC-Alpha Serine/Threonine-Protein Kinase; V-akt Murine Thymoma Viral Oncogene Homolog 1 |
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规格与价格
| 48T 价格 | 1820 |
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| 96T 价格 | 2600 |
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| 96T*5 价格 | 11050 |
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检测参数
| 种属 | Rat |
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| 检测类型 | Sandwich |
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| 灵敏度 | 0.06 ng/mL |
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| 检测范围 | 0.16-10 ng/mL |
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| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Enzyme & Kinase; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKBa in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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